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Real Time SYBR Green Master Mix QPCR P2092 Colourless Appearance

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Real Time SYBR Green Master Mix QPCR P2092 Colourless Appearance

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Brand Name : GDSBio

Model Number : P2091

Certification : /

Place of Origin : China

MOQ : 1Bag

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 100 Bag/Bags Per Day

Delivery Time : 8 work days

Packaging Details : small package or bulk distribute or OEM

Cat. No. : P2091

Specification : 400 rxn/20μl reaction

Appearance : colourless

Group : Real Time PCR Mix

Activity : Pass

Amplification capability : Good

Logo Printing : With Logo Printing

Transport Package : Packing

Production Capacity : 100 Bag/Bags Per Day

Storage Conditions : Store at -20°C

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Cat. No: P2091, 400 rxn/20μl reaction

P2092, 2000 rxn/20μl reaction

SYBR® Green qPCR Mix

SYBR® Green qPCR Mix Real Time PCR Mix P2092

For research use only

Components

Component P2091 P2092
2X SYBR® Green qPCR Mix 1 ml 1 ml × 5
Nuclease-free Water 1 ml 1 ml × 5

Storage

This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.

Description

SYBR® Green qPCR Mix is designed for high-performance, high-throughput real-time PCR. The kit contains Taq DNA Polymerase engineered through a process of molecular evolution. The result is a unique polymerase that specifically designed for qPCR using SYBR® Green I chemistry dye.

2X SYBR® Green qPCR Mix is a convenient premix of the components (except primers and DNA template) that necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green I dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the amount of dsDNA products generated during PCR.

Applications

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

Features

• Compatible with many Real-time systems which not require ROX reference dye

• Hot-start technology brings high specificity and reproducible amplification

Composition of the 2X SYBR Green qPCR Mix

100mM KCl , 5mM MgCl2, 400μM dNTPs, 0.1U/μl Hotstart Taq DNA Polymerase, 1× SYBR® Green I and other optimized buffer components.

Table of Instrument Guide

Instruments Final Conc. of ROX
ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700 500 nM,high ROX
ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000 50 nM,low ROX
Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler No ROX

Real Time SYBR Green Master Mix QPCR P2092 Colourless Appearance

Real Time SYBR Green Master Mix QPCR P2092 Colourless Appearance

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.


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